1nw4

X-ray diffraction
2.2Å resolution

Crystal Structure of Plasmodium falciparum Purine Nucleoside Phosphorylase in complex with ImmH and Sulfate

Released:

Function and Biology Details

Reaction catalysed:
Purine deoxynucleoside + phosphate = purine + 2'-deoxy-alpha-D-ribose 1-phosphate
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
homo hexamer
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PNP_UDP_1 domain-containing protein Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 276 amino acids
Theoretical weight: 30.5 KDa
Source organism: Plasmodium falciparum 3D7
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8I3X4 (Residues: 2-245; Coverage: 100%)
Gene name: PF3D7_0513300
Sequence domains: Phosphorylase superfamily
Structure domains: Rossmann fold

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 86.554Å b: 92.285Å c: 239.64Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.207 0.248
Expression system: Escherichia coli