› 1nvp

X-ray diffraction
2.1Å resolution

HUMAN TFIIA/TBP/DNA COMPLEX

Released:
DOI: 10.2210/pdb1nvp/pdb
PDB entry 1nvp coloured by chain, front view.
PDB entry 1nvp coloured by chain, side view.
PDB entry 1nvp coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Novel interactions between the components of human and yeast TFIIA/TBP/DNA complexes.
Bleichenbacher M, Tan S, Richmond TJ
J Mol Biol 332 783-93 (2003)
PMID: 12972251

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114283 (preferred)
Entry contents:
4 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (6 distinct):
TATA-box-binding protein Chain: A
Molecule details ›
Chain: A
Length: 181 amino acids
Theoretical weight: 20.44 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P20226 (Residues: 159-339; Coverage: 53%)
Gene names: GTF2D1, TBP, TF2D, TFIID
Sequence domains: Transcription factor TFIID (or TATA-binding protein, TBP)
Structure domains: TATA-Binding Protein
Transcription initiation factor IIA alpha chain Chain: B
Molecule details ›
Chain: B
Length: 57 amino acids
Theoretical weight: 6.49 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P52655 (Residues: 2-58; Coverage: 15%)
Gene names: GTF2A1, TF2A1
Structure domains: Helix Hairpins
Transcription initiation factor IIA beta chain Chain: C
Molecule details ›
Chain: C
Length: 76 amino acids
Theoretical weight: 8.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P52655 (Residues: 303-376; Coverage: 20%)
Gene names: GTF2A1, TF2A1
Sequence domains: Transcription factor IIA, alpha/beta subunit
Structure domains: Transcription factor IIA (TFIIA), beta-barrel domain
Transcription initiation factor IIA subunit 2 Chain: D
Molecule details ›
Chain: D
Length: 108 amino acids
Theoretical weight: 12.34 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P52657 (Residues: 2-109; Coverage: 99%)
Gene names: GTF2A2, TF2A2
Sequence domains:
Structure domains:
5'-D(*GP*GP*GP*GP*GP*GP*GP*CP*TP*AP*TP*AP*AP*AP*AP*GP*G)-3' Chain: E
Molecule details ›
Chain: E
Length: 17 nucleotides
Theoretical weight: 5.38 KDa
5'-D(*CP*CP*TP*TP*TP*TP*AP*TP*AP*GP*CP*CP*CP*CP*CP*CP*C)-3' Chain: F
Molecule details ›
Chain: F
Length: 17 nucleotides
Theoretical weight: 5.03 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P212121
Unit cell:
a: 59.624Å b: 90.884Å c: 125.256Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.229 0.247
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Structural basis of transcription initiation by RNA polymerase II.
Sainsbury et al. (2015)
7 more

14 mentions without citation

Zooming in on Transcription Preinitiation.
Gupta et al. (2016)
13 more