› 1ns2

X-ray diffraction
1.95Å resolution

Crystal structure of galactose mutarotase from Lactococcus lactis mutant E304A complexed with galactose

Released:
DOI: 10.2210/pdb1ns2/pdb
PDB entry 1ns2 coloured by chain, front view.
PDB entry 1ns2 coloured by chain, side view.
PDB entry 1ns2 coloured by chain, top view.
Source organism: Lactococcus lactis
Primary publication:
The catalytic mechanism of galactose mutarotase.
Thoden JB, Kim J, Raushel FM, Holden HM
Protein Sci 12 1051-9 (2003)
PMID: 12717027

Function and Biology Details

Reaction catalysed: 
Alpha-D-glucose = beta-D-glucose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195605 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aldose 1-epimerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 347 amino acids
Theoretical weight: 38.56 KDa
Source organism: Lactococcus lactis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9ZB17 (Residues: 1-339; Coverage: 100%)
Gene name: galM
Sequence domains: Aldose 1-epimerase
Structure domains: Beta-galactosidase; Chain A, domain 5

Ligands and Environments

2 bound ligands:
Ligand GAL 2 x GAL
Ligand NI 1 x NI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 44.8Å b: 76.4Å c: 211.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.163 0.229
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Structure and function of enzymes of the Leloir pathway for galactose metabolism.
Holden et al. (2003)
1 more

4 mentions without citation

The catalytic mechanism of galactose mutarotase.
Thoden et al. (2003)
3 more