› 1nqm

X-ray diffraction
1.7Å resolution

Structure of Savm-W120K, streptavidin mutant

Released:
DOI: 10.2210/pdb1nqm/pdb
PDB entry 1nqm coloured by chain, front view.
PDB entry 1nqm coloured by chain, side view.
PDB entry 1nqm coloured by chain, top view.
Source organism: Streptomyces avidinii
Primary publication:
Dimer-tetramer transition between solution and crystalline states of streptavidin and avidin mutants.
Pazy Y, Eisenberg-Domovich Y, Laitinen OH, Kulomaa MS, Bayer EA, Wilchek M, Livnah O
J Bacteriol 185 4050-6 (2003)
PMID: 12837778

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149694 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Streptavidin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 136 amino acids
Theoretical weight: 14.2 KDa
Source organism: Streptomyces avidinii
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P22629 (Residues: 24-159; Coverage: 85%)
Sequence domains: Avidin family
Structure domains: Avidin-like

Ligands and Environments

1 bound ligand:
Ligand BTN 4 x BTN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P21
Unit cell:
a: 50.433Å b: 100.407Å c: 52.511Å
α: 90° β: 112.12° γ: 90°
R-values:
R R work R free
0.215 0.212 0.24
Expression system: Spodoptera frugiperda

Quick links

Citations

2 review citations

Essentials of biorecognition: the (strept)avidin-biotin system as a model for protein-protein and protein-ligand interaction.
Wilchek et al. (2006)
1 more