1nqh

X-ray diffraction
3.1Å resolution

OUTER MEMBRANE COBALAMIN TRANSPORTER (BTUB) FROM E. COLI, WITH BOUND CALCIUM AND CYANOCOBALAMIN (VITAMIN B12) SUBSTRATE

Released:
Source organism: Escherichia coli
Primary publication:
Substrate-induced transmembrane signaling in the cobalamin transporter BtuB.
Nat. Struct. Biol. 10 394-401 (2003)
PMID: 12652322

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Vitamin B12 transporter BtuB Chain: A
Molecule details ›
Chain: A
Length: 594 amino acids
Theoretical weight: 66.39 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P06129 (Residues: 21-614; Coverage: 100%)
Gene names: JW3938, b3966, bfe, btuB, cer, dcrC
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P3121
Unit cell:
a: 81.687Å b: 81.687Å c: 225.814Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.226 0.224 0.265
Expression system: Escherichia coli