› 1nqh

X-ray diffraction
3.1Å resolution

OUTER MEMBRANE COBALAMIN TRANSPORTER (BTUB) FROM E. COLI, WITH BOUND CALCIUM AND CYANOCOBALAMIN (VITAMIN B12) SUBSTRATE

Released:
DOI: 10.2210/pdb1nqh/pdb
PDB 1nqh coloured by chain and viewed from the front.
PDB 1nqh coloured by chain and viewed from the side.
PDB 1nqh coloured by chain and viewed from the top.
Source organism: Escherichia coli
Primary publication:
Substrate-induced transmembrane signaling in the cobalamin transporter BtuB.
Chimento DP, Mohanty AK, Kadner RJ, Wiener MC
Nat. Struct. Biol. 10 394-401 (2003)
PMID: 12652322

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Vitamin B12 transporter BtuB Chain: A
Molecule details ›
Chain: A
Length: 594 amino acids
Theoretical weight: 66.39 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P06129 (Residues: 21-614; Coverage: 100%)
Gene names: JW3938, b3966, bfe, btuB, cer, dcrC
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand CA 4 x CA
Ligand CNC 1 x CNC
Ligand C8E 6 x C8E
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P3121
Unit cell:
a: 81.687Å b: 81.687Å c: 225.814Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.226 0.224 0.265
Expression system: Escherichia coli

Quick links

Citations

29 review citations

Iron Acquisition Systems of Gram-negative Bacterial Pathogens Define TonB-Dependent Pathways to Novel Antibiotics.
Klebba et al. (2021)
28 more

32 mentions without citation

Genetic and structural determinants on iron assimilation pathways in the plant pathogen Xanthomonas citri subsp. citri and Xanthomonas sp.
Guerra et al. (2020)
31 more