Protein Data Bank in Europe

2 ATP = 2 diphosphate + cyclic di-3',5'-adenylate

L-arginine + 2-oxoglutarate + O(2) = (3S)-3-hydroxy-L-arginine + succinate + CO(2)

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP

Acetyl-CoA + a 2-deoxystreptamine antibiotic = CoA + N(3)-acetyl-2-deoxystreptamine antibiotic

Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides

IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate

Autocatalytic release of the core protein from the N-terminus of the togavirus structural polyprotein by hydrolysis of a -Trp-|-Ser- bond.

RX + glutathione = HX + R-S-glutathione

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine

Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate

Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins

NAD(P)H + O(2) = NAD(P)(+) + H(2)O(2)

Hydrolyzes a Gly-|-Gly bond at its own C-terminus, commonly in the sequence -Tyr-Xaa-Val-Gly-|-Gly, in the processing of the potyviral polyprotein.

2 glutathione + ROOH = glutathione disulfide + H(2)O + ROH

Nucleoside 2',3'-cyclic phosphate + H(2)O = nucleoside 3'-phosphate

TSAVLQ-|-SGFRK-NH(2) and SGVTFQ-|-GKFKK the two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive peptide substrates. The enzyme exhibits a strong preference for substrates containing Gln at P1 position and Leu at P2 position.

Beta-D-ribopyranose = beta-D-ribofuranose

[Amino group carrier protein]-C-terminal-gamma-(L-lysyl)-L-glutamate + H(2)O = [amino group carrier protein]-C-terminal-L-glutamate + L-lysine

Cutin + H(2)O = cutin monomers

Selective hydrolysis of -Xaa-Xaa-|-Yaa- bonds in which each of the Xaa can be either Arg or Lys and Yaa can be either Ser or Ala.

Release of N-terminal proline from a peptide.

H(2)CO(3) = CO(2) + H(2)O

GDP-beta-L-fucose + NADP(+) = GDP-4-dehydro-alpha-D-rhamnose + NADPH

Chorismate = prephenate

NTP + H(2)O = NDP + phosphate

Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.

Selective cleavage of Tyr-|-Gly bond in picornavirus polyprotein.

Hydrolyzes glutaminyl bonds, and activity is further restricted by preferences for the amino acids in P6 - P1' that vary with the species of potyvirus, e.g. Glu-Xaa-Xaa-Tyr-Xaa-Gln-|-(Ser or Gly) for the enzyme from tobacco etch virus. The natural substrate is the viral polyprotein, but other proteins and oligopeptides containing the appropriate consensus sequence are also cleaved.

ATP + H(2)O = ADP + phosphate

(R)-10-hydroxystearate = oleate + H(2)O

(R)-3-hydroxybutanoate + NAD(+) = acetoacetate + NADH

Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.

(1a) (2R,3S)-3-isopropylmalate = 2-isopropylmaleate + H(2)O

(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)

A 3'-ribonucleotide + H(2)O = a ribonucleoside + phosphate

N-carbamoylputrescine + H(2)O = putrescine + CO(2) + NH(3)

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)

5,10-methylenetetrahydrofolate + glycine + H(2)O = tetrahydrofolate + L-serine

ADP-alpha-D-glucose + D-glucose 6-phosphate = ADP + alpha,alpha-trehalose 6-phosphate

L-lysine + NADPH + O(2) = N(6)-hydroxy-L-lysine + NADP(+) + H(2)O

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides

Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.

Endohydrolysis of RNA in RNA/DNA hybrids. Three different cleavage modes: 1. sequence-specific internal cleavage of RNA. Human immunodeficiency virus type 1 and Moloney murine leukemia virus enzymes prefer to cleave the RNA strand one nucleotide away from the RNA-DNA junction. 2. RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end. 3. DNA 3'-end directed cleavage 15-20 nucleotides away from the primer terminus.

4 Fe(2+) + 4 H(+) + O(2) = 4 Fe(3+) + 2 H(2)O

(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP(+) = 3-oxoacyl-[acyl-carrier-protein] + NADPH

S-adenosyl-L-methionine + a catechol = S-adenosyl-L-homocysteine + a guaiacol

3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

(R)-pantoate + NADP(+) = 2-dehydropantoate + NADPH

A beta-lactam + H(2)O = a substituted beta-amino acid

Diphosphate + H(2)O = 2 phosphate

4 benzenediol + O(2) = 4 benzosemiquinone + 2 H(2)O

2 glutathione + dehydroascorbate = glutathione disulfide + ascorbate

6-phospho-D-gluconate + NADP(+) = D-ribulose 5-phosphate + CO(2) + NADPH

Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil

(1a) L-cysteine + [enzyme]-cysteine = L-alanine + [enzyme]-S-sulfanylcysteine

5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH