› 1np7

X-ray diffraction
1.9Å resolution

Crystal Structure Analysis of Synechocystis sp. PCC6803 cryptochrome

Released:
DOI: 10.2210/pdb1np7/pdb
PDB 1np7 coloured by chain and viewed from the front.
PDB 1np7 coloured by chain and viewed from the side.
PDB 1np7 coloured by chain and viewed from the top.
Source organism: Synechocystis sp. PCC 6803
Primary publication:
Identification of a new cryptochrome class. Structure, function, and evolution.
Brudler R, Hitomi K, Daiyasu H, Toh H, Kucho K, Ishiura M, Kanehisa M, Roberts VA, Todo T, Tainer JA, Getzoff ED
Mol. Cell 11 59-67 (2003)
PMID: 12535521

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cryptochrome DASH Chains: A, B
Molecule details ›
Chains: A, B
Length: 489 amino acids
Theoretical weight: 57.11 KDa
Source organism: Synechocystis sp. PCC 6803
Expression system: Escherichia coli
UniProt:
  • Canonical: P77967 (Residues: 38-489; Coverage: 92%)
Gene names: cry, phr, phrB, sll1629
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 3 x SO4
Ligand FAD 2 x FAD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: C2
Unit cell:
a: 126.284Å b: 89.477Å c: 121.687Å
α: 90° β: 120.46° γ: 90°
R-values:
R R work R free
0.209 0.208 0.233
Expression system: Escherichia coli

Quick links

Citations

46 review citations

Photoreaction Mechanisms of Flavoprotein Photoreceptors and Their Applications.
Iwata et al. (2021)
45 more

6 mentions without citation

Magnetic field effects in flavoproteins and related systems.
Evans et al. (2013)
5 more