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X-ray diffraction
2.5Å resolution

CRYSTAL STRUCTURE OF MURG:GLCNAC COMPLEX

Released:
DOI: 10.2210/pdb1nlm/pdb
PDB 1nlm coloured by chain and viewed from the front.
PDB 1nlm coloured by chain and viewed from the side.
PDB 1nlm coloured by chain and viewed from the top.
Source organism: Escherichia coli
Primary publication:
Crystal structure of the MurG:UDP-GlcNAc complex reveals common structural principles of a superfamily of glycosyltransferases.
Hu Y, Chen L, Ha S, Gross B, Falcone B, Walker D, Mokhtarzadeh M, Walker S
Proc. Natl. Acad. Sci. U.S.A. 100 845-9 (2003)
PMID: 12538870

Function and Biology Details

Reaction catalysed: 
UDP-N-acetylglucosamine + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = UDP + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 364 amino acids
Theoretical weight: 39.06 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P17443 (Residues: 2-355; Coverage: 100%)
Gene names: JW0088, b0090, murG
Sequence domains:
Structure domains: Glycogen Phosphorylase B;

Ligands and Environments

2 bound ligands:
Ligand UD1 2 x UD1
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P212121
Unit cell:
a: 66.703Å b: 83.651Å c: 146.245Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.226 0.283
Expression system: Escherichia coli

Quick links

Citations

28 review citations

Structural characterization of the O-GlcNAc cycling enzymes: insights into substrate recognition and catalytic mechanisms.
Joiner et al. (2019)
27 more

21 mentions without citation

Glycoscience@Synchrotron: Synchrotron radiation applied to structural glycoscience.
Pérez et al. (2017)
20 more