1nl0

X-ray diffraction
2.2Å resolution

Crystal structure of human factor IX Gla domain in complex of an inhibitory antibody, 10C12

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
hetero hexamer
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
anti-factor IX antibody, 10C12, chain L Chain: L
Molecule details ›
Chain: L
Length: 213 amino acids
Theoretical weight: 22.63 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
anti-factor IX antibody, 10C12, chain H Chain: H
Molecule details ›
Chain: H
Length: 224 amino acids
Theoretical weight: 23.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Coagulation factor IXa light chain Chain: G
Molecule details ›
Chain: G
Length: 51 amino acids
Theoretical weight: 7.12 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P00740 (Residues: 47-91; Coverage: 10%)
Gene name: F9

Ligands and Environments

2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: C2
Unit cell:
a: 105.089Å b: 71.614Å c: 90.867Å
α: 90° β: 119.14° γ: 90°
R-values:
R R work R free
0.235 0.233 0.27
Expression systems:
  • Escherichia coli
  • Not provided