Structure analysis

A BACILLUS DNA POLYMERASE I PRODUCT COMPLEX BOUND TO A GUANINE-THYMINE MISMATCH AFTER FIVE ROUNDS OF PRIMER EXTENSION, FOLLOWING INCORPORATION OF DCTP, DGTP, DTTP, AND DATP.

X-ray diffraction
1.8Å resolution
Assembly composition:
hetero trimer (preferred)
Entry contents: 1 distinct polypeptide molecule
2 distinct DNA molecules

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero trimer
Accessible surface area: 29507.97 Å2
Buried surface area: 6041.34 Å2
Dissociation area: 1,557.53 Å2
Dissociation energy (ΔGdiss): 28.9 kcal/mol
Dissociation entropy (TΔSdiss): 11.06 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-114264

Macromolecules

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Name: DNA PRIMER STRAND
Representative chains: B
Source organism: Geobacillus stearothermophilus [1422]
Expression system: Not provided
Length: 15 nucleotides
Theoretical weight: 4.59 KDa

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Name: DNA TEMPLATE STRAND
Representative chains: C
Source organism: Geobacillus stearothermophilus [1422]
Expression system: Not provided
Length: 15 nucleotides
Theoretical weight: 4.6 KDa

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