1njw

X-ray diffraction
1.9Å resolution

GUANINE-THYMINE MISMATCH AT THE POLYMERASE ACTIVE SITE

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-114250 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (4 distinct):
DNA polymerase I Chain: A
Molecule details ›
Chain: A
Length: 580 amino acids
Theoretical weight: 66.17 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5KWC1 (Residues: 299-878; Coverage: 66%)
Gene names: GK2730, polA
Sequence domains:
Structure domains:
DNA PRIMER STRAND Chain: B
Molecule details ›
Chain: B
Length: 11 nucleotides
Theoretical weight: 3.4 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Not provided
DNA TEMPLATE STRAND Chain: C
Molecule details ›
Chain: C
Length: 15 nucleotides
Theoretical weight: 4.57 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Not provided

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC, FRU
2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 87.95Å b: 93.685Å c: 104.992Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.232 0.209 0.24
Expression systems:
  • Escherichia coli
  • Not provided