1nhc

X-ray diffraction
1.7Å resolution

Structural insights into the processivity of endopolygalacturonase I from Aspergillus niger

Released:

Function and Biology Details

Reaction catalysed:
(1,4-alpha-D-galacturonosyl)(n+m) + H(2)O = (1,4-alpha-D-galacturonosyl)(n) + (1,4-alpha-D-galacturonosyl)(m)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (4 distinct):
Endopolygalacturonase I Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 336 amino acids
Theoretical weight: 34.89 KDa
Source organism: Aspergillus niger
Expression system: Aspergillus niger
UniProt:
  • Canonical: P26213 (Residues: 33-368; Coverage: 96%)
Gene names: pg1, pga1, pgaI
Structure domains: Single-stranded right-handed beta-helix, Pectin lyase-like

Ligands and Environments

Carbohydrate polymer : NEW Components: MAN, NAG, BMA
Carbohydrate polymer : NEW Components: MAN, NAG
Carbohydrate polymer : NEW Components: NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P1
Unit cell:
a: 68.012Å b: 84.13Å c: 96.035Å
α: 114.32° β: 98° γ: 89.75°
R-values:
R R work R free
0.176 0.175 0.209
Expression system: Aspergillus niger