1nc1

X-ray diffraction
2Å resolution

Crystal structure of E. coli MTA/AdoHcy nucleosidase complexed with 5'-methylthiotubercidin (MTH)

Released:

Function and Biology Details

Reaction catalysed:
5'-deoxyadenosine + H(2)O = 5-deoxy-D-ribose + adenine
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 242 amino acids
Theoretical weight: 25.49 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0AF12 (Residues: 1-232; Coverage: 100%)
Gene names: JW0155, b0159, mtn, mtnN, pfs, yadA
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P212121
Unit cell:
a: 51.3Å b: 69.3Å c: 127.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.19 0.223
Expression system: Escherichia coli BL21