1nbu

X-ray diffraction
1.6Å resolution

7,8-Dihydroneopterin Aldolase Complexed with Product From Mycobacterium Tuberculosis

Released:

Function and Biology Details

Reactions catalysed:
7,8-dihydroneopterin = 6-hydroxymethyl-7,8-dihydropterin + glycolaldehyde
7,8-dihydroneopterin = 7,8-dihydromonapterin
7,8-dihydroneopterin + O(2) = 7,8-dihydroxanthopterin + formate + glycolaldehyde
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Dihydroneopterin aldolase Chains: A, E, F, G, H
Molecule details ›
Chains: A, E, F, G, H
Length: 119 amino acids
Theoretical weight: 13.25 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WNC5 (Residues: 1-119; Coverage: 90%)
Gene names: MTCY07H7B.15, Rv3607c, folB
Sequence domains: Dihydroneopterin aldolase
Structure domains: GTP Cyclohydrolase I, domain 2
Dihydroneopterin aldolase Chains: B, D
Molecule details ›
Chains: B, D
Length: 119 amino acids
Theoretical weight: 13.21 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WNC5 (Residues: 1-119; Coverage: 90%)
Gene names: MTCY07H7B.15, Rv3607c, folB
Sequence domains: Dihydroneopterin aldolase
Structure domains: GTP Cyclohydrolase I, domain 2
Dihydroneopterin aldolase Chain: C
Molecule details ›
Chain: C
Length: 119 amino acids
Theoretical weight: 13.12 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WNC5 (Residues: 1-119; Coverage: 90%)
Gene names: MTCY07H7B.15, Rv3607c, folB
Sequence domains: Dihydroneopterin aldolase
Structure domains: GTP Cyclohydrolase I, domain 2

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P21
Unit cell:
a: 78.56Å b: 85.15Å c: 74.79Å
α: 90° β: 112.69° γ: 90°
R-values:
R R work R free
0.177 0.166 0.257
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli