1n9m

X-ray diffraction
1.6Å resolution

Streptavidin Mutant S27A with Biotin at 1.6A Resolution

Released:

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Streptavidin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 127 amino acids
Theoretical weight: 13.27 KDa
Source organism: Streptomyces avidinii
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P22629 (Residues: 37-163; Coverage: 80%)
Structure domains: Avidin-like

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21
Unit cell:
a: 58.43Å b: 63.2Å c: 74.82Å
α: 90° β: 92.8° γ: 90°
R-values:
R R work R free
0.159 0.148 0.207
Expression system: Escherichia coli BL21