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Analysis of the effect of microgravity on protein crystal quality: the case of a myoglobin triple mutant.

Miele AE, Federici L, Sciara G, Draghi F, Brunori M, Vallone B
Acta Crystallogr D Biol Crystallogr 59 982-8 (2003)
Related entries: 1n9h, 1n9i, 1n9x, 1naz

Cited: 8 times
EuropePMC logo PMID: 12777759

Abstract

Crystals of the Met derivative of the sperm whale myoglobin triple mutant Mb-YQR [L(B10)Y, H(E7)Q and T(E10)R] were grown under microgravity conditions and on earth by vapour diffusion. A comparison of crystal quality after complete data collection and processing shows how microgravity-grown crystals diffract to better resolution and lead to considerably improved statistics for X-ray diffraction data compared with crystals grown on earth under the same conditions. The same set of experiments was reproduced on two different Spacelab missions (ISS 6A and ISS 8A) in 2001 and 2002. The structure of this mutant myoglobin, refined using data collected at ELETTRA (Trieste, Italy) from both kinds of crystals, shows that X-ray diffraction from microgravity-grown crystals leads to better defined electron-density maps as well as improved geometrical quality of the refined model. Improvement of the stereochemical parameters of a protein structure is fundamental to quantitative analysis of its function and dynamics and hence to thorough understanding of the molecular mechanisms of action.

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  1. finDr: A web server for in silico D-peptide ligand identification. Engel H, Guischard F, Krause F, Nandy J, Kaas P, Höfflin N, Köhn M, Kilb N, Voigt K, Wolf S, Aslan T, Baezner F, Hahne S, Ruckes C, Weygant J, Zinina A, Akmeriç EB, Antwi EB, Dombrovskij D, Franke P, Lesch KL, Vesper N, Weis D, Gensch N, Di Ventura B, Öztürk MA. Synth Syst Biotechnol 6 402-413 (2021)
  2. Water stabilizes an alternate turn conformation in horse heart myoglobin. Bronstein A, Marx A. Sci Rep 13 6094 (2023)


Reviews citing this publication (1)

  1. Lessons from crystals grown in the Advanced Protein Crystallisation Facility for conventional crystallisation applied to structural biology. Vergara A, Lorber B, Sauter C, Giegé R, Zagari A. Biophys Chem 118 102-112 (2005)

Articles citing this publication (5)

  1. Role and regulation of sigma S in general resistance conferred by low-shear simulated microgravity in Escherichia coli. Lynch SV, Brodie EL, Matin A. J Bacteriol 186 8207-8212 (2004)
  2. Sampling of the native conformational ensemble of myoglobin via structures in different crystalline environments. Kondrashov DA, Zhang W, Aranda R, Stec B, Phillips GN. Proteins 70 353-362 (2008)
  3. Structure of a high-resolution crystal form of human triosephosphate isomerase: improvement of crystals using the gel-tube method. Kinoshita T, Maruki R, Warizaya M, Nakajima H, Nishimura S. Acta Crystallogr Sect F Struct Biol Cryst Commun 61 346-349 (2005)
  4. Molecular Dynamic Simulation of Space and Earth-Grown Crystal Structures of Thermostable T1 Lipase Geobacillus zalihae Revealed a Better Structure. Ishak SNH, Aris SNAM, Halim KBA, Ali MSM, Leow TC, Kamarudin NHA, Masomian M, Rahman RNZRA. Molecules 22 E1574 (2017)
  5. Changes of Thermostability, Organic Solvent, and pH Stability in Geobacillus zalihae HT1 and Its Mutant by Calcium Ion. Ishak SNH, Masomian M, Kamarudin NHA, Ali MSM, Leow TC, Rahman RNZRA. Int J Mol Sci 20 E2561 (2019)


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