› 1n70

X-ray diffraction
1.6Å resolution

The Crystal Structure of Nitrite Reductase Mutant His287Ala from Rhodobacter Sphaeroides

Released:
DOI: 10.2210/pdb1n70/pdb
PDB entry 1n70 coloured by chain, front view.
PDB entry 1n70 coloured by chain, side view.
PDB entry 1n70 coloured by chain, top view.
Source organism: Cereibacter sphaeroides
Entry authors: Guo H, Olesen K, Shapliegh J, Sjolin L

Function and Biology Details

Reaction catalysed: 
Nitric oxide + H(2)O + ferricytochrome c = nitrite + ferrocytochrome c + 2 H(+)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo trimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176048 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Copper-containing nitrite reductase Chain: A
Molecule details ›
Chain: A
Length: 334 amino acids
Theoretical weight: 36.33 KDa
Source organism: Cereibacter sphaeroides
Expression system: Escherichia coli
UniProt:
  • Canonical: Q53239 (Residues: 41-374; Coverage: 97%)
Gene names: Rsph17025_1595, nirK
Sequence domains:
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

2 bound ligands:
Ligand CU 2 x CU
Ligand MG 3 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I711
Spacegroup: R3
Unit cell:
a: 71.395Å b: 71.395Å c: 146.179Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.174 0.174 0.202
Expression system: Escherichia coli

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