1n5a

X-ray diffraction
2.85Å resolution

Crystal structure of the Murine class I Major Histocompatibility Complex of H-2DB, B2-Microglobulin, and A 9-Residue immunodominant peptide epitope gp33 derived from LCMV

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-135025 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
H-2 class I histocompatibility antigen, D-B alpha chain Chains: A, D, G, J
Molecule details ›
Chains: A, D, G, J
Length: 276 amino acids
Theoretical weight: 32.09 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P01899 (Residues: 25-300; Coverage: 82%)
Gene name: H2-D1
Sequence domains:
Structure domains:
Beta-2-microglobulin Chains: B, E, H, K
Molecule details ›
Chains: B, E, H, K
Length: 99 amino acids
Theoretical weight: 11.7 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P01887 (Residues: 21-119; Coverage: 100%)
Gene name: B2m
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Stable signal peptide Chains: C, F, I, L
Molecule details ›
Chains: C, F, I, L
Length: 9 amino acids
Theoretical weight: 1.05 KDa
Source organism: Mammarenavirus choriomeningitidis
Expression system: Not provided
UniProt:
  • Canonical: Q9QDK7 (Residues: 33-41; Coverage: 2%)
Gene name: GPC

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I711
Spacegroup: P21
Unit cell:
a: 91.998Å b: 122.657Å c: 99.18Å
α: 90° β: 103.34° γ: 90°
R-values:
R R work R free
0.253 0.251 0.279
Expression systems:
  • Escherichia coli
  • Not provided