› 1n4c

Solution NMR

NMR Structure of the J-Domain and Clathrin Substrate Binding Domain of Bovine Auxilin

Released:
DOI: 10.2210/pdb1n4c/pdb
PDB entry 1n4c coloured by chain, ensemble of 20 models, front view.
PDB entry 1n4c coloured by chain, ensemble of 20 models, side view.
PDB entry 1n4c coloured by chain, ensemble of 20 models, top view.
Source organism: Bos taurus
Primary publication:
Structure of the functional fragment of auxilin required for catalytic uncoating of clathrin-coated vesicles.
Gruschus JM, Han CJ, Greener T, Ferretti JA, Greene LE, Eisenberg E
Biochemistry 43 3111-9 (2004)
PMID: 15023062

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173296 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Auxilin Chain: A
Molecule details ›
Chain: A
Length: 182 amino acids
Theoretical weight: 20.59 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q27974 (Residues: 737-910; Coverage: 19%)
Gene name: DNAJC6
Structure domains: DnaJ domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: distance geometry simulated annealing
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Not all J domains are created equal: implications for the specificity of Hsp40-Hsp70 interactions.
Hennessy et al. (2005)
3 more

2 mentions without citation

Experimentally biased model structure of the Hsc70/auxilin complex: substrate transfer and interdomain structural change.
Gruschus et al. (2004)
1 more