1n3c

X-ray diffraction
2.7Å resolution

Structural and biochemical exploration of a critical amino acid in human 8-oxoguanine glycosylase

Released:

Function and Biology Details

Reaction catalysed:
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
N-glycosylase/DNA lyase Chain: A
Molecule details ›
Chain: A
Length: 317 amino acids
Theoretical weight: 35.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O15527 (Residues: 12-325; Coverage: 91%)
  • Best match: O15527-5 (Residues: 12-249)
Gene names: MMH, MUTM, OGG1, OGH1
Sequence domains:
Structure domains:
DNA complement strand Chain: B
Molecule details ›
Chain: B
Length: 15 nucleotides
Theoretical weight: 4.64 KDa
Source organism: Homo sapiens
Expression system: Not provided
8-oxoG-containing DNA Chain: C
Molecule details ›
Chain: C
Length: 15 nucleotides
Theoretical weight: 4.56 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P6522
Unit cell:
a: 92.255Å b: 92.255Å c: 210.6Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.225 0.225 0.273
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided