X-ray diffraction
1.9Å resolution

Crystal Structure Analysis of TM0872, a Putative SAM-dependent Methyltransferase, Complexed with SAM


Function and Biology Details

Reaction catalysed:
S-adenosyl-L-methionine + cytosine(1402) in 16S rRNA = S-adenosyl-L-homocysteine + N(4)-methylcytosine(1402) in 16S rRNA 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Ribosomal RNA small subunit methyltransferase H Chains: A, B
Molecule details ›
Chains: A, B
Length: 301 amino acids
Theoretical weight: 35.38 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli BL21(DE3)
  • Canonical: Q9WZX6 (Residues: 1-299; Coverage: 100%)
Gene names: TM_0872, mraW, rsmH
Sequence domains: MraW methylase family
Structure domains:

Ligands and Environments

Cofactor: Ligand SAM 2 x SAM
1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 5ID-B
Spacegroup: P213
Unit cell:
a: 133.96Å b: 133.96Å c: 133.96Å
α: 90° β: 90° γ: 90°
R R work R free
0.206 0.205 0.226
Expression system: Escherichia coli BL21(DE3)