X-ray diffraction
2.6Å resolution

Crystal structure of the N1A mutant of human group IIA phospholipase A2


Function and Biology Details

Reaction catalysed:
Phosphatidylcholine + H(2)O = 1-acylglycerophosphocholine + a carboxylate
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Phospholipase A2, membrane associated Chain: A
Molecule details ›
Chain: A
Length: 124 amino acids
Theoretical weight: 13.9 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P14555 (Residues: 21-144; Coverage: 100%)
Gene names: PLA2B, PLA2G2A, PLA2L, RASF-A
Sequence domains: Phospholipase A2
Structure domains: Phospholipase A2 domain

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS
Spacegroup: P6122
Unit cell:
a: 74.79Å b: 74.79Å c: 88.891Å
α: 90° β: 90° γ: 120°
R R work R free
0.242 0.242 0.32
Expression system: Escherichia coli BL21(DE3)