› 1n1z

X-ray diffraction
2.3Å resolution

(+)-Bornyl Diphosphate Synthase: Complex with Mg and pyrophosphate

Released:
DOI: 10.2210/pdb1n1z/pdb
PDB 1n1z coloured by chain and viewed from the front.
PDB 1n1z coloured by chain and viewed from the side.
PDB 1n1z coloured by chain and viewed from the top.
Source organism: Salvia officinalis
Primary publication:
Bornyl diphosphate synthase: structure and strategy for carbocation manipulation by a terpenoid cyclase.
Whittington DA, Wise ML, Urbansky M, Coates RM, Croteau RB, Christianson DW
Proc. Natl. Acad. Sci. U.S.A. 99 15375-80 (2002)
PMID: 12432096

Function and Biology Details

Reactions catalysed: 
Geranyl diphosphate = (+)-bornyl diphosphate
Geranyl diphosphate = (+)-camphene + diphosphate
Geranyl diphosphate = (+)-alpha-pinene + diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
(+)-bornyl diphosphate synthase, chloroplastic Chains: A, B
Molecule details ›
Chains: A, B
Length: 549 amino acids
Theoretical weight: 64.04 KDa
Source organism: Salvia officinalis
Expression system: Escherichia coli
UniProt:
  • Canonical: O81192 (Residues: 50-598; Coverage: 92%)
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MG 6 x MG
Ligand BTB 2 x BTB
Ligand POP 2 x POP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P212121
Unit cell:
a: 101.21Å b: 117.75Å c: 120.62Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.213 0.244
Expression system: Escherichia coli

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Citations

22 review citations

Current understanding and biotechnological application of the bacterial diterpene synthase CotB2.
Driller et al. (2019)
21 more

3 mentions without citation

Insights into diterpene cyclization from structure of bifunctional abietadiene synthase from Abies grandis.
Zhou et al. (2012)
2 more