PDBe 1mzw

X-ray diffraction
2Å resolution

Crystal structure of a U4/U6 snRNP complex between human spliceosomal cyclophilin H and a U4/U6-60K peptide


Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Peptidyl-prolyl cis-trans isomerase H Chain: A
Molecule details ›
Chain: A
Length: 177 amino acids
Theoretical weight: 19.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
  • Canonical: O43447 (Residues: 1-177; Coverage: 100%)
Gene names: CYP20, CYPH, PPIH
Sequence domains: Cyclophilin type peptidyl-prolyl cis-trans isomerase/CLD
Structure domains: Cyclophilin-like
U4/U6 small nuclear ribonucleoprotein Prp4 Chain: B
Molecule details ›
Chain: B
Length: 31 amino acids
Theoretical weight: 3.5 KDa
Source organism: Homo sapiens
Expression system: Not provided
  • Canonical: O43172 (Residues: 107-137; Coverage: 6%)
Gene names: PRP4, PRPF4
Sequence domains: pre-mRNA processing factor 4 (PRP4) like

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS FR591
Spacegroup: P21
Unit cell:
a: 44.369Å b: 59.526Å c: 47.04Å
α: 90° β: 110.65° γ: 90°
R R work R free
0.195 0.195 0.212
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided