Solution NMR

NMR structure of kinase-interacting FHA domain of kinase associated protein phosphatase, KAPP in Arabidopsis


Function and Biology Details

Reaction catalysed:
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Protein phosphatase 2C 70 Chain: A
Molecule details ›
Chain: A
Length: 139 amino acids
Theoretical weight: 14.89 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
  • Canonical: P46014 (Residues: 180-313; Coverage: 23%)
Gene names: At5g19280, F7K24.30, KAPP, RAG1, T24G5.3
Sequence domains: FHA domain
Structure domains: Tumour Suppressor Smad4

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 91%
Refinement method: torsion angle dynamics and simulated annealing in cartesian space and residual dipolar couplings
Chemical shifts: BMR5841   BMR6474  
Expression system: Escherichia coli