› 1mvw

Electron Microscopy
70Å resolution

MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE

Released:
DOI: 10.2210/pdb1mvw/pdb
PDB 1mvw coloured by chain and viewed from the front.
PDB 1mvw coloured by chain and viewed from the side.
PDB 1mvw coloured by chain and viewed from the top.
Source organisms:
Primary publication:
Molecular modeling of averaged rigor crossbridges from tomograms of insect flight muscle.
Chen LF, Winkler H, Reedy MK, Reedy MC, Taylor KA
J. Struct. Biol. 138 92-104
PMID: 12160705
Related structures: EMD-1001

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
Non-polymer only 32-mer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Myosin heavy chain, skeletal muscle, adult Chains: A, D, G, J, M, P
Molecule details ›
Chains: A, D, G, J, M, P
Length: 840 amino acids
Theoretical weight: 96.63 KDa
Source organism: Gallus gallus
UniProt:
  • Canonical: P13538 (Residues: 5-844; Coverage: 43%)
Sequence domains:
Myosin regulatory light chain 2, skeletal muscle isoform Chains: B, E, H, K, N, Q
Molecule details ›
Chains: B, E, H, K, N, Q
Length: 145 amino acids
Theoretical weight: 16.06 KDa
Source organism: Gallus gallus
UniProt:
  • Canonical: P02609 (Residues: 21-164; Coverage: 86%)
Gene name: MYLPF
Myosin light chain 3, skeletal muscle isoform Chains: C, F, I, L, O, R
Molecule details ›
Chains: C, F, I, L, O, R
Length: 147 amino acids
Theoretical weight: 16.06 KDa
Source organism: Gallus gallus
UniProt:
  • Canonical: P02605 (Residues: 4-150; Coverage: 98%)
Sequence domains: EF-hand domain pair
Actin, alpha skeletal muscle Chains: 1, 2, 3, 4, 5, 6, 7, 8, 9, V, W, X, Y, Z
Molecule details ›
Chains: 1, 2, 3, 4, 5, 6, 7, 8, 9, V, W, X, Y, Z
Length: 375 amino acids
Theoretical weight: 41.86 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P68135 (Residues: 3-377; Coverage: 100%)
Gene names: ACTA, ACTA1
Sequence domains: Actin

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MLY 270 x MLY

Experiments and Validation Details

Entry percentile scores
Resolution: 70Å
Relevant EMDB volumes: EMD-1001

Quick links

Citations

9 review citations

Insights into Actin-Myosin Interactions within Muscle from 3D Electron Microscopy.
Taylor et al. (2019)
8 more

3 mentions without citation

Probing myosin structural conformation in vivo by second-harmonic generation microscopy.
Nucciotti et al. (2010)
2 more