X-ray diffraction
2.5Å resolution

Monoclinic form of Trypanosoma cruzi trans-sialidase, in complex with 3-deoxy-2,3-dehydro-N-acetylneuraminic acid (DANA)and lactose


Function and Biology Details

Reaction catalysed:
Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Sialidase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 648 amino acids
Theoretical weight: 71.39 KDa
Source organism: Trypanosoma cruzi
Expression system: Escherichia coli
  • Canonical: Q26966 (Residues: 2-635; Coverage: 99%)
Sequence domains: BNR repeat-like domain
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC, GAL
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P21
Unit cell:
a: 65.222Å b: 133.374Å c: 90.606Å
α: 90° β: 91.41° γ: 90°
R R work R free
0.194 0.191 0.253
Expression system: Escherichia coli