Solution NMR

Solution structure of the 2nd PHD domain from Mi2b

Source organism: Homo sapiens
Primary publication:
Engineering a protein scaffold from a PHD finger.
Structure 11 803-13 (2003)
PMID: 12842043

Function and Biology Details

Reaction catalysed:
ATP + H(2)O = ADP + phosphate
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Chromodomain-helicase-DNA-binding protein 4 Chain: A
Molecule details ›
Chain: A
Length: 61 amino acids
Theoretical weight: 6.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
  • Canonical: Q14839 (Residues: 446-501; Coverage: 3%)
Gene name: CHD4
Sequence domains: PHD-finger
Structure domains: Zinc/RING finger domain, C3HC4 (zinc finger)

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing, molecular dynamics, torsion angle dynamics
Chemical shifts: BMR5555  
Expression system: Escherichia coli BL21(DE3)