1ml9

X-ray diffraction
1.98Å resolution

Structure of the Neurospora SET domain protein DIM-5, a histone lysine methyltransferase

Released:

Function and Biology Details

Reaction catalysed:
(1a) S-adenosyl-L-methionine + a [histone H3]-L-lysine(9) = S-adenosyl-L-homocysteine + a [histone H3]-N(6)-methyl-L-lysine(9)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Histone-lysine N-methyltransferase, H3 lysine-9 specific dim-5 Chain: A
Molecule details ›
Chain: A
Length: 302 amino acids
Theoretical weight: 34.11 KDa
Source organism: Neurospora crassa
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8X225 (Residues: 30-331; Coverage: 91%)
Gene names: 29E8.110, NCU04402, dim-5
Sequence domains:
Structure domains: SET domain

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P212121
Unit cell:
a: 36.73Å b: 81.56Å c: 101.27Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.205 0.258
Expression system: Escherichia coli BL21