› 1mjm

X-ray diffraction
2.2Å resolution

METHIONINE APOREPRESSOR MUTANT (Q44K) COMPLEXED TO HALF OF THE CONSENSUS OPERATOR SEQUENCE

Released:
DOI: 10.2210/pdb1mjm/pdb
PDB entry 1mjm coloured by chain, front view.
PDB entry 1mjm coloured by chain, side view.
PDB entry 1mjm coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Direct and indirect readout in mutant Met repressor-operator complexes.
Garvie CW, Phillips SE
Structure 8 905-14 (2000)
PMID: 10986458

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114185 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Met repressor Chains: A, B
Molecule details ›
Chains: A, B
Length: 104 amino acids
Theoretical weight: 12.03 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A8U6 (Residues: 2-105; Coverage: 99%)
Gene names: JW3909, b3938, metJ
Sequence domains: Met Apo-repressor, MetJ
Structure domains: MET Apo-Repressor, subunit A
HALF CONSENSUS DNA OPERATOR DUPLEX Chains: C, D
Molecule details ›
Chains: C, D
Length: 10 nucleotides
Theoretical weight: 3.05 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 39.96Å b: 64Å c: 114.47Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.227 0.282
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

Protein flexibility in the light of structural alphabets.
Craveur et al. (2015)
15 other articles

7 mentions without citation

DNA conformations and their sequence preferences.
Svozil et al. (2008)
6 more