1mj3

X-ray diffraction
2.1Å resolution

Crystal Structure Analysis of rat enoyl-CoA hydratase in complex with hexadienoyl-CoA

Released:

Function and Biology Details

Reaction catalysed:
(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Enoyl-CoA hydratase, mitochondrial Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 260 amino acids
Theoretical weight: 28.26 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P14604 (Residues: 31-290; Coverage: 90%)
Gene name: Echs1
Sequence domains: Enoyl-CoA hydratase/isomerase
Structure domains:

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X26C
Spacegroup: P212121
Unit cell:
a: 76.877Å b: 95.198Å c: 249.429Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.172 0.23
Expression system: Escherichia coli