1miw

X-ray diffraction
3Å resolution

Crystal structure of Bacillus stearothermophilus CCA-adding enzyme in complex with ATP

Released:

Function and Biology Details

Reaction catalysed:
(1a) a tRNA precursor + CTP = a tRNA with a 3' cytidine end + diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CCA-adding enzyme Chains: A, B
Molecule details ›
Chains: A, B
Length: 404 amino acids
Theoretical weight: 45.9 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7SIB1 (Residues: 1-404; Coverage: 100%)
Gene name: cca
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-ID-B
Spacegroup: P3121
Unit cell:
a: 105.632Å b: 105.632Å c: 184.159Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.231 0.228 0.263
Expression system: Escherichia coli