› 1miv

X-ray diffraction
3.5Å resolution

Crystal structure of Bacillus stearothermophilus CCA-adding enzyme

Released:
DOI: 10.2210/pdb1miv/pdb
PDB entry 1miv coloured by chain, front view.
PDB entry 1miv coloured by chain, side view.
PDB entry 1miv coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
Crystal structures of the Bacillus stearothermophilus CCA-adding enzyme and its complexes with ATP or CTP.
Li F, Xiong Y, Wang J, Cho HD, Tomita K, Weiner AM, Steitz TA
Cell 111 815-24 (2002)
PMID: 12526808

Function and Biology Details

Reaction catalysed: 
(1a) a tRNA precursor + CTP = a tRNA with a 3' cytidine end + diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181940 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CCA-adding enzyme Chains: A, B
Molecule details ›
Chains: A, B
Length: 404 amino acids
Theoretical weight: 45.9 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7SIB1 (Residues: 1-404; Coverage: 100%)
Gene name: cca
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
1 modified residue:
Ligand MSE 20 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P3121
Unit cell:
a: 105.861Å b: 105.861Å c: 183.658Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.293 0.289 0.328
Expression system: Escherichia coli

Quick links

Citations

13 review citations

Expanding the genetic code.
Wang et al. (2006)
12 more

7 mentions without citation

Evolution of tRNA nucleotidyltransferases: a small deletion generated CC-adding enzymes.
Neuenfeldt et al. (2008)
6 more