1mhp

X-ray diffraction
2.8Å resolution

Crystal structure of a chimeric alpha1 integrin I-domain in complex with the Fab fragment of a humanized neutralizing antibody

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Integrin alpha-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 192 amino acids
Theoretical weight: 21.63 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P18614 (Residues: 169-360; Coverage: 17%)
Gene name: Itga1
Sequence domains: von Willebrand factor type A domain
Structure domains: von Willebrand factor, type A domain
Fab fragment, heavy chain Chains: H, X
Molecule details ›
Chains: H, X
Length: 219 amino acids
Theoretical weight: 23.14 KDa
Source organism: Mus musculus
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins
FAB FRAGMENT, light chain Chains: L, Y
Molecule details ›
Chains: L, Y
Length: 212 amino acids
Theoretical weight: 23.2 KDa
Source organism: Mus musculus
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P65
Unit cell:
a: 255.09Å b: 255.09Å c: 38.64Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.239 0.213 0.272
Expression systems:
  • Escherichia coli
  • Cricetulus griseus