1mgw

X-ray diffraction
2Å resolution

Crystal structure of RNase Sa3, cytotoxic microbial ribonuclease

Released:

Function and Biology Details

Reaction catalysed:
(1a) (RNA) containing guanosine = (RNA fragment)-3'-guanosine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA fragment)
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Guanyl-specific ribonuclease Sa3 Chain: A
Molecule details ›
Chain: A
Length: 99 amino acids
Theoretical weight: 11.06 KDa
Source organism: Kitasatospora aureofaciens
Expression system: Escherichia coli
UniProt:
  • Canonical: P30289 (Residues: 43-141; Coverage: 94%)
Gene name: rnaSA3
Sequence domains: ribonuclease
Structure domains: Microbial ribonucleases

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X31
Spacegroup: P3121
Unit cell:
a: 64.72Å b: 64.72Å c: 69.57Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.155 0.154 0.212
Expression system: Escherichia coli