› 1mdy

X-ray diffraction
2.8Å resolution

CRYSTAL STRUCTURE OF MYOD BHLH DOMAIN BOUND TO DNA: PERSPECTIVES ON DNA RECOGNITION AND IMPLICATIONS FOR TRANSCRIPTIONAL ACTIVATION

Released:
DOI: 10.2210/pdb1mdy/pdb
PDB entry 1mdy coloured by chain, front view.
PDB entry 1mdy coloured by chain, side view.
PDB entry 1mdy coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Crystal structure of MyoD bHLH domain-DNA complex: perspectives on DNA recognition and implications for transcriptional activation.
Ma PC, Rould MA, Weintraub H, Pabo CO
Cell 77 451-9 (1994)
PMID: 8181063

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114177 (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct DNA molecule
Macromolecules (3 distinct):
Myoblast determination protein 1 Chain: A
Molecule details ›
Chain: A
Length: 68 amino acids
Theoretical weight: 8.06 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P10085 (Residues: 102-166; Coverage: 20%)
Gene names: Myod, Myod1
Sequence domains: Helix-loop-helix DNA-binding domain
Structure domains: Helix-loop-helix DNA-binding domain
Myoblast determination protein 1 Chains: B, C, D
Molecule details ›
Chains: B, C, D
Length: 62 amino acids
Theoretical weight: 7.27 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P10085 (Residues: 105-166; Coverage: 20%)
Gene names: Myod, Myod1
Sequence domains: Helix-loop-helix DNA-binding domain
Structure domains: Helix-loop-helix DNA-binding domain
DNA (5'-D(*TP*CP*AP*AP*CP*AP*GP*CP*TP*GP*TP*TP*GP*A)-3') Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 14 nucleotides
Theoretical weight: 4.28 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21212
Unit cell:
a: 222.8Å b: 70.8Å c: 30Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.253 0.253 0.33

Quick links

Citations

34 review citations

Proneural genes and the specification of neural cell types.
Bertrand et al. (2002)
33 more

29 mentions without citation

An overview of the basic helix-loop-helix proteins.
Jones S. (2004)
28 more