PDBe 1mdm

X-ray diffraction
2.8Å resolution

INHIBITED FRAGMENT OF ETS-1 AND PAIRED DOMAIN OF PAX5 BOUND TO DNA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Paired box protein Pax-5 Chain: A
Molecule details ›
Chain: A
Length: 149 amino acids
Theoretical weight: 16.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q02548 (Residues: 1-149; Coverage: 38%)
Gene name: PAX5
Sequence domains: 'Paired box' domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
Protein C-ets-1 Chain: B
Molecule details ›
Chain: B
Length: 161 amino acids
Theoretical weight: 18.68 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P27577 (Residues: 280-440; Coverage: 37%)
Gene names: Ets-1, Ets1
Sequence domains: Ets-domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
PAX5/ETS BINDING SITE ON THE MB-1 PROMOTER Chain: C
Molecule details ›
Chain: C
Length: 26 nucleotides
Theoretical weight: 8.04 KDa
PAX5/ETS BINDING SITE ON THE MB-1 PROMOTER Chain: D
Molecule details ›
Chain: D
Length: 26 nucleotides
Theoretical weight: 7.94 KDa

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21212
Unit cell:
a: 77.27Å b: 171.23Å c: 44.71Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.252 0.259 0.31
Expression system: Escherichia coli BL21(DE3)