X-ray diffraction
2Å resolution

Corynebacterium 2,5-DKGR A and Phe 22 replaced with Tyr (F22Y), Lys 232 replaced with Gly (K232G), Arg 238 replaced with His (R238H)and Ala 272 replaced with Gly (A272G)in presence of NADH cofactor


Function and Biology Details

Reaction catalysed:
2-dehydro-L-gulonate + NADP(+) = 2,5-didehydro-D-gluconate + NADPH
Biochemical function:
Cellular component:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Entry contents:
1 distinct polypeptide molecule
2,5-diketo-D-gluconic acid reductase A Chain: A
Molecule details ›
Chain: A
Length: 278 amino acids
Theoretical weight: 30.06 KDa
Source organism: Corynebacterium sp.
Expression system: Escherichia coli
  • Canonical: P06632 (Residues: 1-278; Coverage: 100%)
Gene name: dkgA
Sequence domains: Aldo/keto reductase family
Structure domains: NADP-dependent oxidoreductase domain

Ligands and Environments

Cofactor: Ligand NAD 1 x NAD
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU, RIGAKU RUH2R
Spacegroup: C2
Unit cell:
a: 111.95Å b: 55.24Å c: 51.54Å
α: 90° β: 111.66° γ: 90°
R R work R free
0.214 0.213 0.261
Expression system: Escherichia coli