1m9h

X-ray diffraction
2Å resolution

Corynebacterium 2,5-DKGR A and Phe 22 replaced with Tyr (F22Y), Lys 232 replaced with Gly (K232G), Arg 238 replaced with His (R238H)and Ala 272 replaced with Gly (A272G)in presence of NADH cofactor

Released:

Function and Biology Details

Reaction catalysed:
2-dehydro-L-gulonate + NADP(+) = 2,5-didehydro-D-gluconate + NADPH
Biochemical function:
Cellular component:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2,5-diketo-D-gluconic acid reductase A Chain: A
Molecule details ›
Chain: A
Length: 278 amino acids
Theoretical weight: 30.06 KDa
Source organism: Corynebacterium sp.
Expression system: Escherichia coli
UniProt:
  • Canonical: P06632 (Residues: 1-278; Coverage: 100%)
Gene name: dkgA
Sequence domains: Aldo/keto reductase family
Structure domains: NADP-dependent oxidoreductase domain

Ligands and Environments


Cofactor: Ligand NAD 1 x NAD
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU, RIGAKU RUH2R
Spacegroup: C2
Unit cell:
a: 111.95Å b: 55.24Å c: 51.54Å
α: 90° β: 111.66° γ: 90°
R-values:
R R work R free
0.214 0.213 0.261
Expression system: Escherichia coli