X-ray diffraction
2.4Å resolution

Crystal Structure Of Methanobacterium Thermoautotrophicum Nicotinamide Mononucleotide Adenylyltransferase Mutant R136A complexed with NAD


Function and Biology Details

Reaction catalysed:
ATP + nicotinamide ribonucleotide = diphosphate + NAD(+)
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Nicotinamide-nucleotide adenylyltransferase Chain: A
Molecule details ›
Chain: A
Length: 181 amino acids
Theoretical weight: 20.48 KDa
Source organism: Methanothermobacter thermautotrophicus
Expression system: Escherichia coli BL21(DE3)
  • Canonical: O26253 (Residues: 1-178; Coverage: 100%)
Gene name: MTH_150
Sequence domains: Cytidylyltransferase-like
Structure domains: HUPs

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P6322
Unit cell:
a: 91.418Å b: 91.418Å c: 107.755Å
α: 90° β: 90° γ: 120°
R R work R free
0.225 0.225 0.265
Expression system: Escherichia coli BL21(DE3)