1m5o

X-ray diffraction
2.2Å resolution

Transition State Stabilization by a Catalytic RNA

Released:
Source organism: Homo sapiens
Primary publication:
Transition state stabilization by a catalytic RNA.
Science 298 1421-4 (2002)
PMID: 12376595

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct RNA molecules
Macromolecules (3 distinct):
U1 small nuclear ribonucleoprotein A Chains: C, F
Molecule details ›
Chains: C, F
Length: 100 amino acids
Theoretical weight: 11.5 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P09012 (Residues: 1-100; Coverage: 36%)
Gene name: SNRPA
Sequence domains: RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain)
Structure domains: Alpha-Beta Plaits
RNA SUBSTRATE Chains: A, D
Molecule details ›
Chains: A, D
Length: 21 nucleotides
Theoretical weight: 6.68 KDa
Source organism: Homo sapiens
Expression system: Not provided
RNA HAIRPIN RIBOZYME Chains: B, E
Molecule details ›
Chains: B, E
Length: 92 nucleotides
Theoretical weight: 29.81 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: C2
Unit cell:
a: 259.38Å b: 44.22Å c: 102.5Å
α: 90° β: 106.3° γ: 90°
R-values:
R R work R free
0.229 0.229 0.264
Expression systems:
  • Escherichia coli
  • Not provided