› 1m5i

X-ray diffraction
2Å resolution

Crystal Structure of the coiled coil region 129-250 of the tumor suppressor gene product APC

Released:
DOI: 10.2210/pdb1m5i/pdb
PDB entry 1m5i coloured by chain, front view.
PDB entry 1m5i coloured by chain, side view.
PDB entry 1m5i coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The coiled coil region (amino acids 129-250) of the tumor suppressor protein adenomatous polyposis coli (APC). Its structure and its interaction with chromosome maintenance region 1 (Crm-1).
Tickenbrock L, Cramer J, Vetter IR, Muller O
J Biol Chem 277 32332-8 (2002)
PMID: 12070164

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150339 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenomatous polyposis coli protein Chain: A
Molecule details ›
Chain: A
Length: 125 amino acids
Theoretical weight: 14.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P25054 (Residues: 126-250; Coverage: 4%)
Gene names: APC, DP2.5
Sequence domains: Adenomatous polyposis coli tumour suppressor protein
Structure domains: Helix hairpin bin

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P3221
Unit cell:
a: 46.047Å b: 46.047Å c: 113.299Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.262 0.257 0.309
Expression system: Escherichia coli BL21

Quick links

Citations

4 review citations

The adenomatous polyposis coli protein: the Achilles heel of the gut epithelium.
Näthke IS. (2004)
3 more

5 mentions without citation

Sequence and structural analyses of nuclear export signals in the NESdb database.
Xu et al. (2012)
4 more