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Structure of the Tsg101 UEV domain in complex with a HIV-1 PTAP "late domain" peptide, DYANA Ensemble

Solution NMR
PDB entry 1m4p coloured by chain, ensemble of 20 models, front view.
PDB entry 1m4p coloured by chain, ensemble of 20 models, side view.
PDB entry 1m4p coloured by chain, ensemble of 20 models, top view.
Source organisms:
Assembly composition:
hetero dimer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Gag protein p6 domain-containing protein and Tumor susceptibility gene 101 protein
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Multimeric state: hetero dimer
Accessible surface area: 9682.88 Å2
Buried surface area: 1060.55 Å2
Dissociation area: 530.27 Å2
Dissociation energy (ΔGdiss): 2.56 kcal/mol
Dissociation entropy (TΔSdiss): 7.17 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-189275

Macromolecules

Tumor susceptibility gene 101 protein
Chain: A
Length: 145 amino acids
Theoretical weight: 16.63 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q99816 (Residues: 1-145; Coverage: 37%)
Gene name: TSG101
Pfam: UEV domain
InterPro:
CATH: Ubiquitin Conjugating Enzyme
SCOP: UEV domain
Tumor susceptibility gene 101 protein in PDB entry 1m4p, assembly 1, front view.
Tumor susceptibility gene 101 protein in PDB entry 1m4p, assembly 1, side view.
Tumor susceptibility gene 101 protein in PDB entry 1m4p, assembly 1, top view.
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Gag protein p6 domain-containing protein
Chain: B
Length: 9 amino acids
Theoretical weight: 966 Da
Source organism: Human immunodeficiency virus 1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9IF21 (Residues: 27-35; Coverage: 13%)
Gene name: pol
Gag protein p6 domain-containing protein in PDB entry 1m4p, assembly 1, front view.
Gag protein p6 domain-containing protein in PDB entry 1m4p, assembly 1, side view.
Gag protein p6 domain-containing protein in PDB entry 1m4p, assembly 1, top view.
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