1m3h

X-ray diffraction
2.05Å resolution

Crystal Structure of Hogg1 D268E Mutant with Product Oligonucleotide

Released:
Source organism: Homo sapiens

Function and Biology Details

Reaction catalysed:
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
3 distinct DNA molecules
Macromolecules (4 distinct):
N-glycosylase/DNA lyase Chain: A
Molecule details ›
Chain: A
Length: 317 amino acids
Theoretical weight: 35.66 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O15527 (Residues: 12-325; Coverage: 91%)
Gene names: MMH, MUTM, OGG1, OGH1
Sequence domains:
Structure domains:
5'-D(P*GP*GP*TP*AP*GP*AP*CP*CP*TP*GP*GP*AP*CP*GP*C)-3' Chain: B
Molecule details ›
Chain: B
Length: 15 nucleotides
Theoretical weight: 4.64 KDa
Source organism: Homo sapiens
Expression system: Not provided
5'-D(P*GP*CP*GP*TP*CP*CP*AP*(DDX))-3' Chain: C
Molecule details ›
Chain: C
Length: 8 nucleotides
Theoretical weight: 2.28 KDa
Source organism: Homo sapiens
Expression system: Not provided
5'-D(P*GP*TP*CP*TP*AP*CP*C)-3' Chain: D
Molecule details ›
Chain: D
Length: 7 nucleotides
Theoretical weight: 2.07 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P6522
Unit cell:
a: 92.345Å b: 92.345Å c: 211.345Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.239 0.239 0.282
Expression systems:
  • Escherichia coli
  • Not provided