› 1m2i

X-ray diffraction
1.8Å resolution

Crystal structure of E44A/E56A mutant of cytochrome b5

Released:
DOI: 10.2210/pdb1m2i/pdb
PDB entry 1m2i coloured by chain, front view.
PDB entry 1m2i coloured by chain, side view.
PDB entry 1m2i coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Structures of Cytochrome b5 Mutated at the Charged Surface-Residues and Their Interactions with Cytochrome c
Wu J, Wang Y-H, Gan J-H, Wang W-H, Sun B-Y, Huang Z-X, Xia Z-X
Chin. J. Chem. 20 1225-1234 (2002)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132303 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome b5 Chain: A
Molecule details ›
Chain: A
Length: 82 amino acids
Theoretical weight: 9.36 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P00171 (Residues: 8-89; Coverage: 61%)
Gene names: CYB5, CYB5A
Sequence domains: Cytochrome b5-like Heme/Steroid binding domain
Structure domains: Cytochrome b5-like heme/steroid binding domain

Ligands and Environments

1 bound ligand:
Ligand HEM 1 x HEM
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: C2
Unit cell:
a: 70.693Å b: 40.49Å c: 39.199Å
α: 90° β: 111.43° γ: 90°
R-values:
R R work R free
0.193 0.193 0.232
Expression system: Escherichia coli

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