1m1y

X-ray diffraction
3.2Å resolution

Chemical Crosslink of Nitrogenase MoFe Protein and Fe Protein

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Nitrogenase molybdenum-iron protein alpha chain Chains: A, C, I, K
Molecule details ›
Chains: A, C, I, K
Length: 491 amino acids
Theoretical weight: 55.23 KDa
Source organism: Azotobacter vinelandii
UniProt:
  • Canonical: P07328 (Residues: 2-492; Coverage: 100%)
Gene name: nifD
Sequence domains: Nitrogenase component 1 type Oxidoreductase
Structure domains: Nitrogenase molybdenum iron protein domain
Nitrogenase molybdenum-iron protein beta chain Chains: B, D, J, L
Molecule details ›
Chains: B, D, J, L
Length: 522 amino acids
Theoretical weight: 59.4 KDa
Source organism: Azotobacter vinelandii
UniProt:
  • Canonical: P07329 (Residues: 2-523; Coverage: 100%)
Gene name: nifK
Sequence domains: Nitrogenase component 1 type Oxidoreductase
Structure domains:
Nitrogenase iron protein 1 Chains: E, F, G, H, M, N, O, P
Molecule details ›
Chains: E, F, G, H, M, N, O, P
Length: 289 amino acids
Theoretical weight: 31.42 KDa
Source organism: Azotobacter vinelandii
UniProt:
  • Canonical: P00459 (Residues: 2-290; Coverage: 100%)
Gene name: nifH1
Sequence domains: 4Fe-4S iron sulfur cluster binding proteins, NifH/frxC family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P212121
Unit cell:
a: 113.267Å b: 214.937Å c: 320.466Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.279 0.279 0.329