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Crystal structure of T4-lysozyme generated from synthetic coding DNA expressed in Escherichia coli.

Rose DR, Phipps J, Michniewicz J, Birnbaum GI, Ahmed FR, Muir A, Anderson WF, Narang S
Protein Eng 2 277-82 (1988)
Cited: 21 times
EuropePMC logo PMID: 3074306

Abstract

The polypeptide produced by expressing a chemically synthesized gene coding for the amino-acid sequence of T4-lysozyme has been crystallized and subjected to X-ray diffraction. The crystal structure has been refined to a standard R-factor of 0.191 for data between 8 and 2 A resolution. The refined model is essentially the same as the well-known structure of wild-type T4-lysozyme determined previously by Matthews et al. (1987). Some small changes in the C-terminal region, which is important in maintaining the folded structure, have been noted. In addition to confirming that the synthetic gene product is very close to the wild type, this structure provides a benchmark for protein engineering experiments on the folding and the catalytic activity of this molecule by the method of gene synthesis.

Reviews - 1lyd mentioned but not cited (1)

  1. Bacteriophage T4 genome. Miller ES, Kutter E, Mosig G, Arisaka F, Kunisawa T, Rüger W. Microbiol Mol Biol Rev 67 86-156, table of contents (2003)

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Articles citing this publication (10)

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  4. What is the average conformation of bacteriophage T4 lysozyme in solution? A domain orientation study using dipolar couplings measured by solution NMR. Goto NK, Skrynnikov NR, Dahlquist FW, Kay LE. J Mol Biol 308 745-764 (2001)
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  8. Interaction-component analysis of the effects of urea and its alkylated derivatives on the structure of T4-lysozyme. Yamamori Y, Matubayasi N. J Chem Phys 146 225103 (2017)
  9. Parametric models to compute tryptophan fluorescence wavelengths from classical protein simulations. Lopez AJ, Martínez L. J Comput Chem 39 1249-1258 (2018)
  10. Site Identification by Ligand Competitive Saturation-Biologics Approach for Structure-Based Protein Charge Prediction. Orr AA, Tao A, Guvench O, MacKerell AD. Mol Pharm 20 2600-2611 (2023)


Related citations provided by authors (2)

  1. Hierarchical Strategy for Protein Folding and Design. Synthesis and Expression of T4 Lysozyme Gene and Two Putative Folding Mutants. Narang SA, Yao F-L, Michniewicz JJ, Dubuc G, Phipps J, Somorjai RL Protein Eng. 1 481- (1987)
  2. Structure of Bacteriophage T4 Lysozyme Refined at 1.7 Angstroms Resolution. Weaver LH, Matthews BW J. Mol. Biol. 193 189- (1987)

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