› 1lxh

Solution NMR

Solution structure of alpha-cobratoxin complexed with a cognate peptide (minimized average structure)

Released:
DOI: 10.2210/pdb1lxh/pdb
PDB 1lxh coloured by chain and viewed from the front.
PDB 1lxh coloured by chain and viewed from the side.
PDB 1lxh coloured by chain and viewed from the top.
Source organisms:
Primary publication:
NMR-based binding screen and structural analysis of the complex formed between alpha-cobratoxin and an 18-mer cognate peptide derived from the alpha 1 subunit of the nicotinic acetylcholine receptor from Torpedo californica.
Zeng H, Hawrot E
J. Biol. Chem. 277 37439-45 (2002)
PMID: 12133834

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Alpha-cobratoxin Chain: A
Molecule details ›
Chain: A
Length: 71 amino acids
Theoretical weight: 7.84 KDa
Source organism: Naja kaouthia
UniProt:
  • Canonical: P01391 (Residues: 1-71; Coverage: 100%)
Structure domains: CD59
Acetylcholine receptor subunit alpha Chain: B
Molecule details ›
Chain: B
Length: 19 amino acids
Theoretical weight: 2.42 KDa
Source organism: Tetronarce californica
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P02710 (Residues: 205-222; Coverage: 4%)
Gene name: CHRNA1

Ligands and Environments

No bound ligands
1 modified residue:
Ligand HSL 1 x HSL

Experiments and Validation Details

Entry percentile scores
Refinement method: distance geometry and simulated annealing
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Nicotinic acetylcholine receptor inhibitors derived from snake and snail venoms.
Dutertre et al. (2017)
4 more

2 mentions without citation

SPA: Short peptide analyzer of intrinsic disorder status of short peptides.
Xue et al. (2010)
1 more