1lwx

X-ray diffraction
2.3Å resolution

AZT DIPHOSPHATE BINDING TO NUCLEOSIDE DIPHOSPHATE KINASE

Released:
Source organism: Dictyostelium discoideum
Primary publication:
X-ray analysis of azido-thymidine diphosphate binding to nucleoside diphosphate kinase.
Proc. Natl. Acad. Sci. U.S.A. 94 7162-5 (1997)
PMID: 9207061

Function and Biology Details

Reaction catalysed:
ATP + nucleoside diphosphate = ADP + nucleoside triphosphate
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nucleoside diphosphate kinase, cytosolic Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 155 amino acids
Theoretical weight: 16.77 KDa
Source organism: Dictyostelium discoideum
Expression system: Escherichia coli
UniProt:
  • Canonical: P22887 (Residues: 1-155; Coverage: 100%)
Gene names: DDB_G0273069, DDB_G0273805, gip17, ndkB, ndkC-1, ndkC-2
Sequence domains: Nucleoside diphosphate kinase
Structure domains: Nucleoside diphosphate kinase-like domain

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LURE BEAMLINE DW32
Spacegroup: P3121
Unit cell:
a: 71.59Å b: 71.59Å c: 152.7Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.216 0.216 0.28
Expression system: Escherichia coli