X-ray diffraction
2.7Å resolution

Crystal structure of the methylimidazole-bound BjFixL heme domain


Function and Biology Details

Reaction catalysed:
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Sensor protein FixL Chain: A
Molecule details ›
Chain: A
Length: 131 amino acids
Theoretical weight: 14.92 KDa
Source organism: Bradyrhizobium japonicum
Expression system: Escherichia coli
  • Canonical: P23222 (Residues: 141-270; Coverage: 26%)
Gene names: bll2760, fixL
Sequence domains: PAS fold
Structure domains: PAS domain

Ligands and Environments

Cofactor: Ligand HEM 1 x HEM
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-D
Spacegroup: R32
Unit cell:
a: 127.02Å b: 127.02Å c: 58.62Å
α: 90° β: 90° γ: 120°
R R work R free
0.201 0.201 0.229
Expression system: Escherichia coli