› 1lsw

X-ray diffraction
2.2Å resolution

Crystal structure of the ferrous BjFixL heme domain

Released:
DOI: 10.2210/pdb1lsw/pdb
PDB entry 1lsw coloured by chain, front view.
PDB entry 1lsw coloured by chain, side view.
PDB entry 1lsw coloured by chain, top view.
Source organism: Bradyrhizobium japonicum
Primary publication:
Structure-based mechanism of O2 sensing and ligand discrimination by the FixL heme domain of Bradyrhizobium japonicum.
Hao B, Isaza C, Arndt J, Soltis M, Chan MK
Biochemistry 41 12952-8 (2002)
PMID: 12390021

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149894 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sensor protein FixL Chain: A
Molecule details ›
Chain: A
Length: 131 amino acids
Theoretical weight: 14.92 KDa
Source organism: Bradyrhizobium japonicum
Expression system: Escherichia coli
UniProt:
  • Canonical: P23222 (Residues: 141-270; Coverage: 26%)
Gene names: bll2760, fixL
Sequence domains: PAS fold
Structure domains: PAS domain

Ligands and Environments


Cofactor: Ligand HEM 1 x HEM
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: R32
Unit cell:
a: 127.57Å b: 127.57Å c: 58.42Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.221 0.221 0.265
Expression system: Escherichia coli

Quick links

Citations

15 review citations

Genetic regulation of biological nitrogen fixation.
Dixon et al. (2004)
14 more

5 mentions without citation

CO-sensing mechanisms.
Roberts et al. (2004)
4 more