› 1ls5

X-ray diffraction
2.8Å resolution

Crystal structure of plasmepsin IV from P. falciparum in complex with pepstatin A

Released:
DOI: 10.2210/pdb1ls5/pdb
PDB entry 1ls5 coloured by chain, front view.
PDB entry 1ls5 coloured by chain, side view.
PDB entry 1ls5 coloured by chain, top view.
Source organisms:
Primary publication:
Novel uncomplexed and complexed structures of plasmepsin II, an aspartic protease from Plasmodium falciparum.
Asojo OA, Gulnik SV, Afonina E, Yu B, Ellman JA, Haque TS, Silva AM
J Mol Biol 327 173-81 (2003)
PMID: 12614616

Function and Biology Details

Reaction catalysed: 
Hydrolysis of the bonds linking certain hydrophobic residues in hemoglobin or globin. Also cleaves small molecules substrates such as Ala-Leu-Glu-Arg-Thr-Phe-|-Phe(NO(2))-Ser-Phe-Pro-Thr.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero octamer
hetero tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-162299 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Plasmepsin IV Chains: A, B
Molecule details ›
Chains: A, B
Length: 328 amino acids
Theoretical weight: 36.99 KDa
Source organism: Plasmodium falciparum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8IM16 (Residues: 122-449; Coverage: 73%)
Gene names: PF3D7_1407800, PMIV
Sequence domains: Eukaryotic aspartyl protease
Structure domains: Acid Proteases
Pepstatin Chains: C, D
Molecule details ›
Chains: C, D
Length: 6 amino acids
Theoretical weight: 686 Da
Source organism: Streptomyces argenteolus subsp. toyonakensis
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P3121
Unit cell:
a: 101.93Å b: 101.93Å c: 123.389Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.22 0.22 0.29
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

7 review citations

Plasmepsins as potential targets for new antimalarial therapy.
Ersmark et al. (2006)
6 more

16 mentions without citation

Microbial and fungal protease inhibitors--current and potential applications.
Sabotič et al. (2012)
15 more